Isolation, Molecular Identification, and Anti-Staphylococcus aureus ATCC 25923 Activity Testing of Endosymbiotic Bacteria from Litopenaeus vannamei
DOI:
https://doi.org/10.33096/jffi.v13i1.w4dbrs33Kata Kunci:
Litopenaeus vannamei, shrimp head waste, endosymbion bacteries, antibacterialAbstrak
L. vannamei, one species of the subphylum Crustacea, which is the main commodity of Indonesian seas because it’s fast growth, high survival rate, adaptability and high selling price. The presence of endosymbiont microscopic organisms that live and develop in creature tissues by advantageous mutualism, moreover has great capacity and movement as antibacterial specialists. This study aims to isolate, molecularly differentiate, and evaluate the antibiotic activity of S. aureus from endosymbiont microorganisms present in L. vannamei head wastes. Shrimp head waste was immunized on supplement agar media to achieve bacterial containment. The growing bacterial colonies were filtered for atomic differentiating evidence and easily observable, minuscule testing. Using the plate dissemination approach, endosymbiont bacterial confines were tested for antibacterial migration (Kirby-Bauer). The inhibition zone formed was observed. Endosymbiont bacterial colonies that have antibacterial movement were recognized molecularly utilizing 16S rRNA primers followed by analysis using the BLAST method and phylogenetic construction using MEGAX software with UPGMA method. Endosymbiont bacterial separate was gotten which had potential movement shown by the arrangement of an restraint zone against S. aureus ATCC 25923. Auxiliary metabolites of endosymbiont microscopic organisms were aged on supplement broth medium and after that tried for antibacterial movement against S. aureus ATCC 25923. There was antibacterial activity at concentrations of 10%, 15% and 20% of secondary metabolite extracts of endosymbiont bacterial isolates with an average diameter of 11.07 mm, 11.97 mm and 13.91 mm. Molecular identification showed that endosymbiont bacterial isolate was closely related to Staphylococcus gallinarum strain RTE-S1 (LC572265).
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